Sugar solution is made transparent embryo and brain




Sugar solution is made transparent embryo and brain

transparent mouse embryo

Neuroscience Group of the Institute of Physical and Chemical Research (RIKEN) in Japan has created a sugary liquid, which makes biological tissue transparent. The aqueous solution was called fructose SeeDB successfully tested on mice.

Scientists have made a mouse embryo and brain transparent, without damaging the samples. They are then visualized neural circuit diagram. This allowed us to see the structure of the brain tissue without cutting into small pieces, which is extremely important for neurobiology.

In fact, RIKEN scientists have created a cleaning agent. It’s hard to believe, but the clarifier is a saturated aqueous solution of fructose with the addition of α-thioglycerol to prevent browning. Before becoming transparent specimen soaked in the solution for three days. All the matter in the refractive index, which has SeeDB (1,490). Have a refractive index similar lipids, fat-like substance in the living cells.

The tissue sample could make out through the two-photon laser microscope after addition of the neural circuit pattern of fluorescent dyes.

Unlike other clarifiers, SeeDB changes neither the chemical composition nor the structure of the tissue, which is very important in the study of the brain. Sugar solution is inexpensive and easy to work with it, the researchers note.

The researchers were able to observe for the first time the nerve fibers connecting the left and right hemisphere of the mouse brain, as well as take pictures of the neurons in the olfactory bulb.


Today, it is the best method of constructing neural maps rodent brain, and tomorrow – man. Technology will help to better understand the human brain and in the future will be used to create complex implants, scientists say.

embryo and brain

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